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Portal Streamlines Multiplexed Cell Engineering

Portal Unlocks Cell Engineering Capabilities
Gene Editing
Deletion, correction, insertions across primary cells
Multiplexed Modifications
Simultaneous delivery of multiple RNA and CRISPR molecules for multi engineered cells
Immune Cell Therapies
Engineering adaptive and innate immune responses across oncology, infectious disease and autoimmunity
iPSCs & Regenerative Medicine
Engineered tissues, disease modeling, drug screening in patient cells

Portal is Developing a Universal Cell Engineering Solution

Portal’s proprietary membrane technology uses mechanical disruption and enables massive parallelization

Portal mechanoporation yields robust multiplex delivery in Unstimulated T Cells

Activated NK cells Boost

50+ partners worldwide, including

Products

Gateway

Research Scale

Benchtop instrument for discovery research

  • 0.5-10M cells per run
  • 50-200 µl volume range
  • Compact benchtop unit that fits in any hood
  • Available now

Galaxy-i

High-Throughput Screening

High throughput scale instrument for screening applications with multi well plates.

  • 96/384-well plate compatibility
  • Integrate with existing automation
  • Ideal for DEL, PROTAC, CRISPR screening
  • Available now

Millibooster

Clinical Manufacturing

GMP-ready clinical production system

  • 1 billion+ cells/minute throughput
  • Attach to existing equipment via tube-weld or luer lock connection
  • 2-100mL volume range
  • Available Now

Cell Engineering Kits

Validated biological workflows — a one stop shop.

Every kit includes: Portal-sourced RNA · Optimized protocols · MicroBooster™ cartridges · Suggested readouts · Reagents

CELL THERAPY

T Cell Functional Enhancement

  • Best suited for: Human T cells (activated or unstimulated), PBMCs
  • Available enhancements: mbIL2, mbIL12, Perforin, Gzmb, B2M and TCR knockouts
  • Measure endogenous intracellular proteins in live cells
  • Use cases: Cytokine armoring, gene KO, persistence enhancement
Download Info
STEM CELL BIOLOGY

iPSC Generation

  • Best suited for: PBMCs & fibroblasts
  • Available enhancements: Oct4, Sox2, Klf4, cMyc RNA
  • Best suited for: Reprogramming, disease modeling
Download Info
IMMUNOLOGY

APC Enhancement

  • Best suited for: PBMCs, dendritic cells
  • Available enhancements: mbIL2, mbIL12, CD86, custom antigen RNA
  • Best suited for: Immune activation, DC engineering, vaccine research
Download Info
Stem cell biology

HSC Enhancement

  • Best suited for: Hematopoietic stem cells
  • Available enhancements: CXCR4, BCL2
  • Best suited for: Engraftment, survival, stemness preservation
Download Info
NEUROSCIENCE

Neuronal Differentiation

  • Best suited for: iPSC-derived neurons, neural progenitors
  • Available enhancements: NGN2, ASCL1, LMX1A, FOXA2, NURR1, ASOs
  • Best suited for: Neuronal differentiation, disease modeling, Parkinson's/ALS
Download Info

Pick your cell and cargo to see the data

All Cells
All Cargos
Publication · 2022 · FRONTIERS IN IMMUNOLOGY
Engineered Red Blood Cells (Activating Antigen Carriers) Drive Potent T Cell Responses and Tumor Regression in Mice

Katarina Blagovic, Carolyne K. Smith, Amritha Ramakrishnan, Lindsay Moore, David R. Soto, Zachary Thompson, Adam P. Stockmann, Sonia Kruszelnicki, Akshi Thakkar, Jason Murray, Sebastian Torres, Bersabel Wondimagegnhu, Roslyn Yi, Maisam Dadgar, Abdul M. Paracha, Claire Page, Louise Clear, Omer A. Chaudhry, Melissa Myint, Devin T. Bridgen, Jonathan B. Gilbert, Katherine J. Seidl, Armon Sharei, Scott Loughhead, Howard Bernstein, and Defne Yarar

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Publication · 2022 · ESMO IMMUNO-ONCOLOGY AND TECHNOLOGY
Cell Squeeze®: Driving more effective CD8 T cell activation through cytosolic antigen delivery

Jong Chul Park, Howard Bernstein, Scott Loughhead, Ricardo Zwirtes, Julia

Jennings, Valeria Nicolini, Christian Klein, Laura Codarri Deak, Pablo Umana,

Christine Trumpfheller, Armon Sharei

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Publication · 2022 · FRONTIERS IN IMMUNOLOGY
Engineered RBCs Encapsulating Antigen Induce Multi-Modal Antigen-Specific Tolerance and Protect Against Type 1 Diabetes

Colin J. Raposo, Judith D. Cserny, Gloria Serena, Jonathan N. Chow, Patricia Cho, Hanyang Liu, David Kotler, Armon Sharei, Howard Bernstein, and Shinu John

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Publication · 2022 · JOURNAL OF IMMUNOLOGY
Microfluidic Squeezing Enables MHC Class I Antigen Presentation by Diverse Immune Cells to Elicit CD8+ T Cell Responses with Antitumor Activity

Matthew G. Booty, Kelan A. Hlavaty, Adam Stockmann, Howard Bernstein, Armon Sharei, Scott M. Loughhead, Hendrik Knoetgen, Christine Trumpfheller, Pablo Umaña, Ulrich H. von Andrian, Klavs F. Jensen, Robert Langer, Jacques, Eritza Chong, Lucas Pomerance, John T. Gonzalez, Shirley Mao, Megan Heimann, LeeAnn Talarico, Miye K. Jonathan Chow, Tarek Abdeljawad, Harry An, Sophia Liu, Vicente-Suarez, Defne Yarar, Melissa Myint, Amy Merino, Olivia Pryor, Kelly Volk, Katarina Blagovic, Ildefonso, Disha Subramanya, Anita Venkitaraman, Christian Yee, Emrah Ilker Ozay, Carolyne Smith, Lina Tian, Edylle How

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