Portal Enables Robust Cell Engineering and Cell Analytics Across Applications

Universal Delivery System for Cell Engineering

Simple, high throughput mechanical delivery technology. Used standalone or integrated into existing equipment

Transient Cell Modification Library

RNA-based cell enhancements complementing genetic approaches (e.g. cytokines, transcription factors, survival factors, and beyond)

Intracellular Delivery is Critical to Many Research and Clinical Applications

Portal is Developing a Universal Cell Engineering Solution

Portal’s proprietary membrane technology uses mechanical disruption and enables massive parallelization

Portal excels where others struggle, enabling cytosolic access for diverse cell types and cargoes

Status Quo

With Portal

Portal’s delivery mechanism preserves cell function

Cells exposed to electroporation misregulate gene expression

Cells exposed to mechanoporation are more similar to controls

Portal mechanoporation yields robust multiplex delivery in Unstimulated T Cells

Want to see data on another cell type? Just ask!

Cell Engineering Kits

Validated biological workflows — a one stop shop.

Every kit includes: Portal-sourced RNA · Optimized protocols · MicroBooster™ cartridges · Suggested readouts · Reagents

CELL THERAPY

T Cell Functional Enhancement

Best suited for: Human T cells (activated or unstimulated), PBMCs
Available enhancements: mbIL2, mbIL12, Perforin, Gzmb, B2M and TCR knockouts
Measure endogenous intracellular proteins in live cells
Use cases: Cytokine armoring, gene KO, persistence enhancement

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STEM CELL BIOLOGY

iPSC Generation

Best suited for: PBMCs & fibroblasts
Available enhancements: Oct4, Sox2, Klf4, cMyc RNA
Best suited for: Reprogramming, disease modeling

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IMMUNOLOGY

APC Enhancement

Best suited for: PBMCs, dendritic cells
Available enhancements: mbIL2, mbIL12, CD86, custom antigen RNA
Best suited for: Immune activation, DC engineering, vaccine research

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Stem cell biology

HSC Enhancement

Best suited for: Hematopoietic stem cells
Available enhancements: CXCR4, BCL2
Best suited for: Engraftment, survival, stemness preservation

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NEUROSCIENCE

Neuronal Differentiation

Best suited for: iPSC-derived neurons, neural progenitors
Available enhancements: NGN2, ASCL1, LMX1A, FOXA2, NURR1, ASOs
Best suited for: Neuronal differentiation, disease modeling, Parkinson's/ALS

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Drug Discovery Kits

Portal — enabling live-cell assays, target engagement, and compound profiling.

Kits include: Optimized protocols · Portal reagents · Delivery cartridges · Suggested readouts

Impermeable Candidate Validation in Live Cells

Solves: Permeability engineering as a prerequisite for testing cellular activity
Cargo: Your impermeable drug candidate
Validate and test your candidates in live cells, regardless of permeability
e.g. small molecules, macrocycles, peptides

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Live-Cell Protein Detection

Solves: Live-cell context lost to lysis, and target labeling that requires cell engineering.
Cargo: Intracellular antibodies (primary & secondary)
Measure endogenous intracellular proteins in live cells
e.g. p-BTK, p-ERK, p-AKT, p-STAT3, p-MET Compatible with LgBiT/HgBiT reagents

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Live-Cell Target Engagement

Solves: Live-cell context lost to lysis and detergents, and the need to create cell-permeable tracers
Cargoes: Your tracers (e.g. NanoBRET) and compounds
Enables live-cell target engagement without lysis; supports impermeable tracers
e.g. kinase inhibitor profiling, degrader engagement

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Controllable Surface Antigen Expression

Solves: Fixed, hard-to-modulate surface antigen expression in cell lines
Cargo: Custom antigen/receptor RNA — single or combinations
Express any antigen on any cell in hours
e.g. CD19+CD3, BCMA+CD3, HER2+CD3

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Direct-to-Assay

Solves: The stable-line bottleneck: infeasible in primary cells, slow and laborious elsewhere
Cargo: Custom RNA encoding tagged POI
Run NanoBRET or LgBiT/HgBiT without stable lines
e.g. VHL-NanoLuc, HgBiT-tagged POI, HA-tagged POI

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